FABRICATION OF A CELL-BASED BIOSENSOR
USING GREEN TAPE CERAMICS WITH HUMAN EMBRYONIC KIDNEY AND SEA URCHIN EGG
CELLS
DeAnna Burns (Mech Eng. & Appl Mech), University
of Pennsylvania
Advisors: H. Bau (Mech Eng) , J. Tanaka (Medicine, Temple U,.), P. Mueller
(BioPhysics, Penn),
J. Van der Spiegel (EE, Penn)
ABSTRACT
Cells are electrically active; thus fabrication of a cell-based biosensor
will contribute much information about how cells respond to different environments.
With such a device, researchers can submit various types of cells to different
forms of stimulus and observe the cells’ reactions. I designed and fabricated
a biosensor out of DuPont Green Tape ceramics and thick film conductor
materials, working with human embryonic kidney cells in order to test the
compatibility of cells with various types of materials. I found that DuPont’s
gold paste conductor is harmful to cells and that pure gold is needed in
order for cells to attach and live on the sensor’s electrodes. Next, I
turned to sea urchin eggs, which are larger and more robust than most cells.
I constructed a device containing channels with two pure gold electrodes
covered with a thin insulating layer of Teflon. A small portion of the
Teflon removed over each electrode provides the necessary small area needed
for an electrode that is used to measure the electrical activity of cells.
Although I was unable to gain many meaningful electrical measurements,
an improved sensor that is constructed out of more compatible materials
should be constructed and used to test a cell’s electrical response to
a change in environment.
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ELECTROMAGNETICALLY
ACTUATED GAS DIVERTING VALVE USING LTCC TAPE
Jason Gillman (EE) – University of Pennsylvania
Advisors: Dr. J.J. Santiago-Aviles, P. Espinoza
ABSTRACT
This research attempted to fabricate a gas-diverting valve using LTCC
technology for the emerging field of meso-scale devices. A photolithographic
process for patterning Low Temperature Co-Fired Ceramic (LTCC) tapes utilizing
DuPont Green Tape? tape coupled with DuPont Riston? 9015, a dry photoresist,
is described in detail. Based on the device design, calculations were made
determining the theoretical resistance and magnetic flux were made. These
numbers will determine the voltage necessary to run the device once built.
Although the device was not constructed since the resolution of the dry
photoresist system was being pushed led to difficulty in etching the tape,
lamination and development techniques of the Riston? were perfected, and
insights into the composition of the tape prior to and after etching were
made. Suggestions for improvements in the process are given, as well as
thoughts on what should be the next step in this research.
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GLUCOSE SENSOR UTILIZING SILICON
PLANAR TECHNOLOGY
Charlotte Martinez (Electrical Engineering) University of Pennsylvania
Advisors: Dr. J.J. Santiago-Aviles, P. Espinoza
ABSTRACT
A glucose sensor using silicon and platinum was designed and fabricated.
The interaction of glucose oxidaise and glucose produces proteins, which
have dielectric properties that produce an electrical output measured through
the bonding pads of a sensor. The sensor consists of two layers of platinum:
one porous layer that serves as an area for the reaction to take place,
and a second layer, that consists of two bonding pads. The sensors were
placed in solutions of different glucose concentration. Their resistance
was measured. It was found that in a solution of less concentration, the
resistance was greater. We also researched non-invasive ways of measuring
glucose. Technologies used included Raman spectroscopy, near-infrared spectroscopy,
photo acoustic spectroscopy and mid-infrared spectroscopy.
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(pdf - 504KB)
GERIATRIC FALL HIP INJURY PREVENTION
DEVICE
Xiomara Feliciano-Diaz (Mechanical Engineering) – Turabo
University, Puerto Rico
Advisor: Dr. Jorge J. Santiago-Aviles
ABSTRACT
Hip fractures can be a life-threatening injury among the
elderly. Annually, thirty million falls are reported, out of which two
hundred and fifty thousand result in hip fractures. Medical costs for these
fractures are estimated between seven and ten billion dollars annually.
However, the number of hip fractures is expected to double in the next
fifty years, which defines the current problem as critical and alarming.
In order to provide a suitable solution to this problem, Dr. Jorge Santiago
from the Electrical Engineering Department at the University of Pennsylvania
has developed a project to build such device. The product will be a belt-like
device that would prevent hip injuries by means of sensors that will recognize
a potentially injurious fall and then trigger an inflatable cushion before
impact.
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(pdf - 278 KB)
DESIGNING A TRI-AXIAL ACCELEROMETER
INTERFACE FOR THE MEASUREMENT OF IMPACT FORCES CAUSED BY ATHLETIC COLLISIONS
Heather Marandola (Electrical Engineering), Swarthmore College
Advisor: Jim Ostrowski
ABSTRACT
This paper describes the design of an accelerometer system to be used
for the transmission of collision forces during the course of a contact
sporting event. Discussed in this paper are basic accelerometer and transmission
principles, the development and results of data acquisition experiments
and the design of three evaluation boards: accelerometer, transmission
and reception. My work has led to the design of preliminary calibration
and display techniques for dual axis accelerometer outputs, and I have
produced evaluation boards which can be utilized in further experimentation.
Though a secure transmission link was not established, progress has been
made in the development of the transmission scheme to be implemented on
the device. Measurement along three axes was not implemented; however,
with minor revisions, the evaluation boards are capable of handling one,
two, three or four axes. The device is not ready to be installed into the
sporting arenas, but some of the principles and design ideas presented
in this paper might be of use in future implementation.
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(pdf - 398 KB)
DESIGNING AN UNDERWATER
EEL-LIKE ROBOT AND DEVELOPING ANGUILLIFORM LOCOMOTION CONTROL
Tamara Knutsen, Harvard University
Advisors: Professor Jim Ostrowski (GRASP Lab), and Kenneth McIsaac (GRASP
Lab)
ABSTRACT
In this report, I investigate the design and development of an
eel-like amphibious robot. After reviewing research on dynamic mobile
robots and control and motion planning for biomimetic robot systems, I
analyzed the prototype created by Kenneth McIsaac and Jim Ostrowski and
redesigned its key mechanical, electrical and communication components.
The significant changes I implemented affected the number of links of the
robot, its waterproof characteristics and the communication design (both
hardware and software). I designed and manufactured the mechanical
components using the state-of-the-art CAD/CAM facilities at the University
of Pennsylvania and assembled and waterproofed the prototype at the GRASP
laboratory. McIsaac and I made the necessary modifications to the
open-loop control of the robot and at the time of writing this report,
the prototype seems to be functioning as expected. The closed loop
control of the robot is still being developed at this time.
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(pdf
- 764KB)
SENSOR FUSION FOR ESTIMATING MOTION
OF A
LEGGED ROBOTIC SOCCER PLAYER
Julie Neiling (Computer Engineering), University of Evansville, Indiana
Advisor: Dr. James Ostrowski
ABSTRACT
This paper describes the investigation of the accelerometers, gyroscopes,
speaker, and microphones on the Sony AIBO, which is a robotic dog. The
purpose is to use these sensors to determine the position of the robot
during a soccer game at RoboCup. The gyroscope generates data used to tell
rotation; accelerometer data is used to calculate distance traveled; audio
communication can be used to pass messages that relay to its position on
the playing field to the other robots. The sensor data will be incorporated
into the robot AI through individual software modules.
The primary emphasis of the paper is the research involved in development
of the audio communication. To accomplish the communication we used various
frequency analysis and signal processing tools to make the robot produce
tones and correctly identify them.
.
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(pdf - 3MB)
SOURCE ANALYSIS AND CELLULAR INTERROGATION USING THE
ELECTROENCEPHALOGRAM
Lauren Berryman (Electrical Engineering), University of Pennsylvania
Advisors: Dr. Nader Engheta, Dr. Edward N. Pugh
Collaborators: Claire Daniele, Lauren Daniele
ABSTRACT
This paper describes the electroencephalographic detection and analysis
of scalp potentials utilizing fMRI brain volume data to design stimuli
that activate specific visual areas and pathways. Our dual purpose was
to re-evaluate current scientific models and to investigate the origin
of neural activity in response to a specified stimulus. Through data collection
and analysis, we tested the widely used three-shell conductivity model
of the head. We concluded that this model is inappropriate for source analysis
due to inherent model flaws and the disagreement of our experimental observations
with model predictions. We approached the problem of dipole current source
analysis by first pinpointing the source location within the visual cortex
using collected metabolic fMRI data. Then, with our stimulus designed to
activate that identified area of cortex, we recorded electromagnetic scalp
distribution patterns. By examining the collected sensory evoked potentials
with Fourier analysis, we found frequency components present in the scalp
recording at twice the stimulus flicker frequency. By tracing frequency
response patterns and identifying corresponding contrast response parameters,
we were able to show that for our stimulus, the magnocellular pathway was
dominant in the flow of visual information from retina to cortex
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(pdf - 2.7 MB)
COMPONENTS OF A CMOS IMAGER FOR
A POLARIZATION-DIFFERENCE CAMERA
Shiva Portonovo (Electrical Engineering), University of Pennsylvania
Advisors: Dr. Jan Van der Spiegel and Dr. Nader Engheta
ABSTRACT
The problem of perceiving objects that are suspended in scattering media
limits the ability of humans to derive information about their environment.
This can be the cause of several important dangers to personal safety,
while it can also prevent people from learning about their surroundings.
>From studying the visual systems of animals that have a better sense of
perception in such media, the concept of polarization-difference imaging
was born. A system that employs this technique uses the polarization properties
of light in novel ways to produce images that detect objects in scattering
media and make surface features discernable. Such a system is a polarization-difference
camera. A polarization-difference camera can consist of a polarization
analyzer, a solid-state imager, and an image display system. The polarization
analyzer separates detected light into orthogonally polarized beams. The
solid-state imager transduces the light signals into electronic signals
and processes the data to produce the polarization-difference output and
other necessary operations. The image display system transforms the data
so that images can be optimally displayed. This report focuses on the design
aspects of the solid-state imager for a polarization-difference camera.
The solid-state imager may be constructed with complementary metal-oxide
semiconductor (CMOS) devices, currently the most widely used variety of
devices in integrated electronic systems. The pixels used in the imager,
which employ active transistors, are known as active pixel sensors. A set
of several designs of components that may be used in the solid-state imager
are described and analyzed. Suggestions for optimization for an integrated
design follow.
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(pdf - 375 KB)
SPARSE CANTOR RING ANTENNA ARRAYS
WITH
NON-UNIFORM ELEMENT SPACING
Frederick U. Diaz, University of Pennsylvania (Electrical Engineering)
NSF-AMP Undergraduate Research Program
Advisors: Dr. Dwight L. Jaggard and Aaron D. Jaggard
ABSTRACT
This research project is based on previous work done by Dwight L. Jaggard
and Aaron D. Jaggard on fractal ring arrays. We utilize fractals in the
fabrication of a class of Cantor ring arrays. Fractal descriptors such
as dimension, stage of growth, and lacunarity are applied in designing
and characterizing these Cantor rings. Using the continuous arrays developed
by Jaggard and Jaggard as a point of reference, we examine the design of
analogous discrete arrays. We examine various ways of periodically and
randomly thinning and building up these arrays azimuthally. The arrays
are compared to their periodic and random counterparts as well as the continuous
case to evaluate performance. Mainbeam quality, sidelobe level, and visible
range are used to rate the arrays. The Cantor ring arrays and the corresponding
array factors are simulated using programs developed in MATLAB. Our goals
include developing fractal arrays that have low sidelobes comparable to
those of periodic arrays while maintaining the robustness of random arrays.
We aim for superior fractal performance at an equal number of elements
as the periodic and random cases, as well as comparable performance using
fewer elements.
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SPIRAL FRACTAL ARRAYS
Héctor E. Dimas - (Electrical Engineering), University of Pennsylvania
NSF-AMPS/SUNFEST Summer Undergraduate Research
Advisors: Dr. Dwight L. Jaggard, Aaron D. Jaggard
ABSTRACT
This paper describes the use of fractal arrangements for the design
of planar antenna arrays. Spiral initiators were used to play the chaos
game, which generated our fractal array. Once these antenna arrays were
produced, the resulting radiated field was calculated using scripts written
in Matlab. Variations on the number of arms and inner circles in our spiral
initiators were tested to characterize which formation would allow for
our fractal array to perform equivalently or better than a set of random
elements placed on a given circular area. The compared random array held
the same number of elements as our spiral array did which was usually 441.
The resultant spiral array behaved much like tapered arrays in that the
concentration of points tended towards the center. The overall behavior
of the fractal array in comparison to the random array was comparable in
terms of directivity and average sidelobe level. Additional attempts to
make the spiral array less tapered allowed for possible characterization
as a “multi-fractal” array by changing the number of points that made up
inner circles of the initiator spiral. These results also proved to be
comparable to random and spiral arrays. Implications of these results and
further exploration needed are discussed.
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(pdf - 2.44 MB)
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